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SPTLC1 Antibody

Images

 
Western Blot: SPTLC1 Antibody [NBP2-56477] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: SPTLC1 Antibody [NBP2-56477] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Independent Antibodies: Analysis using NBP2-56477 (A) shows similar pattern to independent antibody NBP1-81737 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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SPTLC1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPTLC1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 1:100 - 1:250
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SPTLC1 Recombinant Protein Antigen (NBP2-56477PEP)

Reactivity Notes

Mouse 88%, Rat 86%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SPTLC1 Antibody

  • EC 2.3.1.50
  • hereditary sensory neuropathy, type 1
  • HSAN1
  • LBC1
  • LCB 1
  • LCB1
  • LCB1HSAN
  • Long chain base biosynthesis protein 1
  • MGC14645
  • serine C-palmitoyltransferase
  • serine palmitoyltransferase 1
  • serine palmitoyltransferase, long chain base subunit 1
  • Serine-palmitoyl-CoA transferase 1
  • SPT 1
  • SPT1
  • SPT1HSN1
  • SPTI
  • SPTLC1

Background

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SPTLC1