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ISG15 Activating Enzyme/UBE1L Antibody

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Immunocytochemistry/Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [NBP2-55032] - Staining of human cell line RT4 shows localization to nucleoplasm, cytosol & vesicles.
Immunocytochemistry/ Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [NBP2-55032] - Staining of human cell line RT4 shows localization to nucleoplasm, cytosol & vesicles.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ISG15 Activating Enzyme/UBE1L Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBA7
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ISG15 Activating Enzyme/UBE1L Recombinant Protein Antigen (NBP2-55032PEP)

Reactivity Notes

Mouse 81%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ISG15 Activating Enzyme/UBE1L Antibody

  • D8
  • D8UBA1, ubiquitin-activating enzyme E1 homolog B
  • ISG15 Activating Enzyme/UBE1L
  • UBA1B
  • UBA7
  • UBA7, ubiquitin-activating enzyme E1
  • UBE1L
  • UBE2
  • UBE2MGC12713
  • Ubiquitin-activating enzyme 7
  • Ubiquitin-activating enzyme E1 homolog
  • ubiquitin-activating enzyme E1-like
  • ubiquitin-activating enzyme E1-related protein
  • ubiquitin-activating enzyme-2
  • ubiquitin-like modifier activating enzyme 7
  • ubiquitin-like modifier-activating enzyme 7

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ISG15 Activating
Enzyme/UBE1L Antibody
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Bioinformatics

Gene Symbol UBA7