![Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (JE50-55) [NBP3-33075] - Western blot analysis of ISG15 Activating Enzyme/UBE1L on THP-1 cell lysates with Rabbit anti-ISG15 Activating Enzyme/UBE1L antibody (NBP3-33075) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 112 kDa Observed band size: 112 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33075) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33075_rabbit-isg15-activating-enzyme-ube1l-mab-je50-55-28820241419158.jpg "Western Blot: ISG15 Activating Enzyme/UBE1L Antibody (JE50-55) [NBP3-33075] - Western blot analysis of ISG15 Activating Enzyme/UBE1L on THP-1 cell lysates with Rabbit anti-ISG15 Activating Enzyme/UBE1L antibody (NBP3-33075) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 112 kDa Observed band size: 112 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33075) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | JE50-55 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human ISG15 Activating Enzyme/UBE1L aa 1-100 / 1,012. (Uniprot: P41226) |
| Localization | Cytoplasm, cytosol, nucleoplasm, nucleus |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | UBA7 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 112 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBA7 |
![Immunohistochemistry: ISG15 Activating Enzyme/UBE1L Antibody (JE50-55) [NBP3-33075] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ISG15 Activating Enzyme/UBE1L antibody (NBP3-33075) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33075) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33075_rabbit-isg15-activating-enzyme-ube1l-mab-je50-55-29820241435216.jpg "Immunohistochemistry: ISG15 Activating Enzyme/UBE1L Antibody (JE50-55) [NBP3-33075] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ISG15 Activating Enzyme/UBE1L antibody (NBP3-33075) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33075) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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