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IRF4 Antibody (CL14615)

Images

 
Staining of human small intestine shows strong nuclear positivity in lymphoid cells.
Staining of human tonsil shows strong nuclear positivity in lymphoid cells.
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Orthogonal Strategies: Analysis in human tonsil and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Western blot analysis in human cell line SK-MEL-30.
Staining of human skin shows strong nuclear positivity in a subset of lymphoid cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL14615
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

IRF4 Antibody (CL14615) Summary

Immunogen
This antibody was developed against a Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence of Q15306. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (95%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IRF4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IRF4 Antibody (CL14615)

  • interferon regulatory factor 4
  • IRF4
  • IRF-4
  • LSIRF
  • LSIRFMUM1
  • Lymphocyte-specific interferon regulatory factor
  • Multiple myeloma oncogene 1
  • MUM1
  • NF-EM5

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IRF4