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IRAK1 Antibody (3A9)

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Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.
Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Genetic Strategies: Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or ...read more
Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - IRAK1 monoclonal antibody (M02), clone 3A9 Analysis of IRAK1 expression in A-431.
Immunoprecipitation: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal ...read more
ELISA: IRAK1 Antibody (3A9) [H00003654-M02] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, KD
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

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IRAK1 Antibody (3A9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS
Localization
interleukin-1 receptor complex
Specificity
IRAK1 - interleukin-1 receptor-associated kinase 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRAK1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRAK1 Antibody (3A9)

  • EC 2.7.11
  • EC 2.7.11.1
  • Il1rak
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • IRAK-1
  • IRAKPelle homolog
  • Pelle
  • Plpk

Background

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IRAK1
Entrez
OMIM
Uniprot