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Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free

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Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Analysis using the Biotin conjugate of NBP1-43400. Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k ...read more
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (NBP1-43400) (open histogram) ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready
Clone
GoH3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free Summary

Immunogen
The immunogen for this antibody was CD49f.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ITGA6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This GoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26254531)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free

  • CD49 antigen-like family member F
  • CD49f antigen
  • CD49f
  • DKFZp686J01244
  • FLJ18737
  • Integrin alpha 6
  • integrin alpha-6
  • integrin alpha6B
  • integrin, alpha 6
  • ITGA6
  • ITGA6B
  • Platelet gpl
  • VLA-6

Background

The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITGA6