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IL-9 Antibody (623153) [Unconjugated]

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Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB209) or (B) Mouse IgG2B Isotype Control Antibody (MAB0041) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
623153
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB209U-100UG
Alexa Fluor 405
FAB209V-100UG
Alexa Fluor 488
FAB209G-100UG
Alexa Fluor 532
FAB209X
Alexa Fluor 594
FAB209T-100UG
Alexa Fluor 647
FAB209R-100UG
Alexa Fluor 700
FAB209N-100UG
Alexa Fluor 750
FAB209S-100UG
Allophycocyanin
FAB209A
Allophycocyanin
IC209A
Biotin
FAB209B
DyLight 350
FAB209D
DyLight 405
FAB209E
DyLight 488
FAB209K
DyLight 550
FAB209L
DyLight 594
FAB209M
DyLight 650
FAB209W
DyLight 680
FAB209Y
DyLight 755
FAB209Z
FITC
FAB209F
HRP
FAB209H
Janelia Fluor 549
FAB209I
Janelia Fluor 646
FAB209J
PE
FAB209P
PerCP
FAB209C
mFluor Violet 450 SE
FAB209MFV450
mFluor Violet 500 SE
FAB209MFV500
mFluor Violet 610 SE
FAB209MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC209A
Carrier Free
MAB209-SP
Carrier Free
FAB209V-100UG
Carrier Free
FAB209U-100UG
Carrier Free
FAB209T-100UG
Carrier Free
FAB209S-100UG
Carrier Free
FAB209R-100UG
Carrier Free
FAB209N-100UG
Carrier Free
FAB209G-100UG
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IL-9 Antibody (623153) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248
Specificity
Detects human IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat IL-9 or recombinant mouse IL-9 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL9
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-9 Antibody (623153) [Unconjugated]

  • Cytokine P40
  • HP40
  • IL9
  • IL-9
  • IL-9homolog of mouse T cell and mast cell growth factor 40
  • interleukin 9
  • interleukin-9
  • p40 cytokine
  • p40 T-cell and mast cell growth factor
  • P40
  • T-cell growth factor P40

Background

Interleukin-9 (IL-9) is a 14 kDa glycosylated cytokine that is secreted by CD4+ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-9 Antibody (MAB209) (0)

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FAQs for IL-9 Antibody (MAB209) (0)

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Bioinformatics

Gene Symbol IL9
Uniprot