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IL-7R alpha/CD127 Antibody (73N8F2)

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Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.
Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Mouse testis stained with IL-7R antibody at 5 ug/ml.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
73N8F2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25264AF350
Alexa Fluor 405
NBP2-25264AF405
Alexa Fluor 488
NBP2-25264AF488
Alexa Fluor 532
NBP2-25264AF532
Alexa Fluor 594
NBP2-25264AF594
Alexa Fluor 647
NBP2-25264AF647
Alexa Fluor 700
NBP2-25264AF700
Alexa Fluor 750
NBP2-25264AF750
Biotin
NBP2-25264B
DyLight 350
NBP2-25264UV
DyLight 405
NBP2-25264V
DyLight 488
NBP2-25264G
DyLight 550
NBP2-25264R
DyLight 594
NBP2-25249DL594
DyLight 650
NBP2-25264C
DyLight 680
NBP2-25264FR
DyLight 755
NBP2-25264IR
FITC
NBP2-25264F
HRP
NBP2-25264H
Janelia Fluor 549
NBP2-25264JF549
Janelia Fluor 646
NBP2-25264JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-25264
Azide and BSA Free
NBP2-25264SS
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IL-7R alpha/CD127 Antibody (73N8F2) Summary

Immunogen
Amino acids 145-235 of mouse IL-7R / CD127 were used as the immunogen for the antibody.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL7R
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-5 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IL-7R alpha/CD127 Antibody (73N8F2)

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

IL-7R, or CD127, is often used to characterize regulatory T cells or Tregs. By phenotyping, IL-7R is inversely correlated with the high levels of the hallmark Treg transcription factor FoxP3. So lowered expression of IL-7R on the cell surface along with CD25 and CD4 in addition to FoxP3 internal expression is a major and routinely used technique for Treg cell studies. IL-7R residing on the surface enables enrichment and purification of Treg populations. Antibody to IL-7R is useful for phenotyping, identification, enumeration and purification of Tregs (1-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-7R alpha/CD127
Antibody (73N8F2)
NBP2-25249
IL-7R alpha/CD127 Antibody (73N8F2)
Species: Mu
Applications: WB, IHC
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Bioinformatics

Gene Symbol IL7R
Uniprot