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Common gamma Chain/IL-2 R gamma Antibody [Unconjugated]

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Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common gamma Chain/IL‑2 R gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND50 is typically 20-60 µg/mL.
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (30 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF284
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF284-SP
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Common gamma Chain/IL-2 R gamma Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common  gamma  Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785
Specificity
Detects human Common  gamma  Chain/IL‑2 R gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-2 R gamma is observed, less than 10% cross-reactivity with recombinant human (rh) IL-2 R beta is observed, and less than 2% cross-reactivity with rhIL-2 R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL2RG
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<1.0 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 20-60 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF284 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody [Unconjugated]

  • CD132 antigen
  • CD132
  • CIDX
  • combined immunodeficiency, X-linked
  • common cytokine receptor gamma chain
  • Common gamma Chain
  • common gamma-chain
  • cytokine receptor common subunit gamma
  • gamma(c)
  • IL-2 R gamma
  • IL-2 receptor subunit gamma
  • IL2R gamma
  • IL-2R subunit gamma
  • IL2RG
  • IL-2RG
  • IMD4
  • interleukin 2 receptor, gamma
  • Interleukin-2 receptor subunit gamma
  • P64
  • SCIDX
  • SCIDX1
  • severe combined immunodeficiency

Background

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Common gamma Chain/IL-2 R gamma Antibody (AF284)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(3)
IHC-Fr
(1)
Immunoprecipitation
(2)
Western Blot
(2)
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Human
(4)
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Showing Publications 1 - 6 of 6.
Publications using AF284 Applications Species
Jingwei Shi, Xiao Shen, Qi Kang, Xing Yang, Maximilian Denzinger, Marko Kornmann et al. Loss of Interleukin-13-Receptor-Alpha-1 Induces Apoptosis and Promotes EMT in Pancreatic Cancer International Journal of Molecular Sciences 3/26/2022 [PMID: 35409019]
Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity Frontiers in Immunology 1/14/2021 [PMID: 33584648]
Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro. PLoS ONE, 2014-05-12;9(5):e96925. 2014-05-12 [PMID: 24819779] (Flow Cytometry, IHC-Fr, Human) Flow Cytometry, IHC-Fr Human
Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response. J. Biol. Chem., 2010-02-18;285(20):14898-908. 2010-02-18 [PMID: 20167604] (Flow Cytometry, Immunoprecipitation, Western Blot, Human) Flow Cytometry, Immunoprecipitation, Western Blot Human
Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009-06-15;182(12):7389-97. 2009-06-15 [PMID: 19494261] (Immunoprecipitation, Human) Immunoprecipitation Human
Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients. J. Immunol., 2008-06-01;180(11):7221-9. 2008-06-01 [PMID: 18490721] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human

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As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
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Bioinformatics

Gene Symbol IL2RG
Uniprot