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IL-5 Antibody (604312) [Unconjugated]

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Recombinant Feline IL‑5 (Catalog # 1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL‑5 Monoclonal Antibody (Catalog # MAB1795). The ND50 is typically 0.1-0.5 µg/mL.
Recombinant Feline IL‑5 (Catalog # 1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ...read more

Product Details

Summary
Reactivity FeSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
604312
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1795-SP
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IL-5 Antibody (604312) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515
Specificity
Detects feline IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant feline GM-CSF, recombinant equine, canine, mouse, human, or porcine IL-5 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IL5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.5 ug/mL
Application Notes
ELISA Capture: Feline IL-5 Antibody (Catalog # MAB1795)
ELISA Detection: Feline IL-5 Biotinylated Antibody (Catalog # BAM17951)
Standard: Recombinant Feline IL-5 (Catalog # 1795-FL)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (604312) [Unconjugated]

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

Interleukin-5 (IL-5) is a 40 kDa secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the alpha -helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115 amino acid (aa) mature feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding alpha subunit with a signal transducing common beta subunit ( beta c) that is shared with receptor complexes for IL-3 and GM-CSF.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-5 Antibody
(604312) [Unconjugate
MAB1795
IL-5 Antibody (604312) [Unconjugated]
Species: Fe
Applications: B/N, ELISA(Cap)

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Bioinformatics

Gene Symbol IL5
Uniprot