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IL-37/IL-1F7 Antibody [Unconjugated]

 (6 Publications)
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Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 µg/mL LPS for 24 hours and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 10 µg/mL LPS for 3 hours and 200 nM PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-37/IL-1F7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-37/IL-1F7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 µg/mL LPS for 24 hours and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1975
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Catalog# & Formulation Size Price
Carrier Free
AF1975-SP
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IL-37/IL-1F7 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-37/IL-1F7
Lys27-Asp192
Accession # Q9NZH6-2
Specificity
Detects human IL-37/IL-1F7 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) FIL1δ, rhFIL1 epsilon , rhFIL1 eta, rhIL-1 alpha , rhIL-1 beta , rhIL-18, and rhIL-1 H1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL37
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF1975 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-37/IL-1F7 Antibody [Unconjugated]

  • FIL1 zeta
  • FIL1
  • FIL1(ZETA)
  • FIL1ZFIL1 zeta
  • IL-1F7
  • IL-1F7IL-1 zeta
  • IL1H4IL1F7 (canonical product IL-1F7b)
  • IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1)
  • IL-1RP1IL-1X protein
  • IL1RP1interleukin 1 family member 7
  • IL-1X
  • IL37
  • IL-37
  • interleukin 1 family, member 7 (zeta)
  • interleukin 1, zeta
  • interleukin-1 family member 7
  • Interleukin-1 homolog 4
  • interleukin-1 superfamily z
  • Interleukin-1 zeta
  • Interleukin-1-related protein

Background

Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha , IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha , IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta . This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Busfield S.J. et al. (2000) Genomics 66:213.
  4. Kumar S. et al. (2002) Cytokine 18:61.
  5. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  6. Gao, W. et al. (2003) J. Immunol. 170:1077.
  7. Pan, G. et al. (2001) Cytokine 13:1.
  8. Sims, J.E. et al. (2001) Trends Immunol. 22:533.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-37/IL-1F7 Antibody (AF1975)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Detection, IHC-P, Neutralization, Western Blot.


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ELISA Detection
(1)
IHC-P
(1)
Neutralization
(1)
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(2)
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(3)
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Showing Publications 1 - 6 of 6.
Publications using AF1975 Applications Species
A Gritsenko, R Díaz-Pino, G Lopez-Cast NLRP3 inflammasome triggers interleukin-37 release from human monocytes European Journal of Immunology, 2022-04-28;0(0):. 2022-04-28 [PMID: 35429346] (ELISA Detection, Western Blot, Human) ELISA Detection, Western Blot Human
S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation Nat Commun, 2018-09-11;9(1):3686. 9/11/2018 [PMID: 30206230]
Sara McCurdy, Yvonne Baumer, Emma Toulmin, Bog-Hieu Lee, William A. Boisvert Macrophage-Specific Expression of IL-37 in Hyperlipidemic Mice Attenuates Atherosclerosis The Journal of Immunology 2017-11-15 [PMID: 29030487]
M Zhao, Y Hu, J Jin, Y Yu, S Zhang, J Cao, Y Zhai, R Wei, J Shou, W Cai, S Liu, X Yang, GT Xu, J Yang, DB Corry, SB Su, X Liu, T Yang Interleukin 37 promotes angiogenesis through TGF-? signaling Sci Rep, 2017-07-21;7(1):6113. 2017-07-21 [PMID: 28733640] (Western Blot, Human) Western Blot Human
Bulau A, Nold M, Li S, Nold-Petry C, Fink M, Mansell A, Schwerd T, Hong J, Rubartelli A, Dinarello C, Bufler P Role of caspase-1 in nuclear translocation of IL-37, release of the cytokine, and IL-37 inhibition of innate immune responses. Proc Natl Acad Sci U S A, 2014-01-30;111(7):2650-5. 2014-01-30 [PMID: 24481253] (Neutralization, Mouse) Neutralization Mouse
Teng X, Hu Z, Wei X, Wang Z, Guan T, Liu N, Liu X, Ye N, Deng G, Luo C, Huang N, Sun C, Xu M, Zhou X, Deng H, Edwards C, Chen X, Wang X, Cui K, Wei Y, Li J IL-37 ameliorates the inflammatory process in psoriasis by suppressing proinflammatory cytokine production. J Immunol, 2014-01-22;192(4):1815-23. 2014-01-22 [PMID: 24453242] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol IL37
Uniprot