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IL-17/IL-17A ELISA Development Kit

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ELISA: IL-17/IL-17A ELISA Development Kit [NBP3-11745] - Standard curve reference
ELISA: IL-17/IL-17A ELISA Development Kit [NBP3-11745] - Standard curve reference

Product Details

Summary
Reactivity BvSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernatants
Standard Curve Range
1 - 200 pg/mL

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IL-17/IL-17A ELISA Development Kit Summary

Standard Curve Range
1 - 200 pg/mL
Sample Volume
100 uL
Kit Type
ELISA Development Kit
Gene
IL17A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated detection mAb (MT51B8)
  2. Capture mAb (MT49A7)
  3. Recombinant bovine IL-17A ELISA standard
  4. Standard reconstitution buffer
  5. Streptavidin-HRP

Alternate Names for IL-17/IL-17A ELISA Development Kit

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by TCRalpha/beta+ CD4 CD8 thymocytes, as well as by activated CD4+ and CD4+ CD45RO+ memory T cells (1). IL-17 stimulates the expression of TNFalpha, IL-1 beta, IL-6, and IL-8 (2). In addition, IL-17 is involved in the induction of inducible nitric oxide synthase and cyclooxygenase 2 in chondrocytes (3). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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IL-17/IL-17A ELISA
Development Kit
NBP3-11745
IL-17/IL-17A ELISA Development Kit
Species: Bv
Applications: ELISA

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Product General Protocols

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FAQs for IL-17/IL-17A Kit (NBP3-11745). (Showing 1 - 2 of 2 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Contact Information

Product PDFs

  Manual - IL-17/IL-17A ELISA Development Kit
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol IL17A