Home » IL-12 R beta 2 » IL-12 R beta 2 Antibodies » IL-12 R beta 2 Antibody
Novus Biologicals products are now on bio-techne.com

IL-12 R beta 2 Antibody

 (2 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for IL-12 R beta 2 Antibody (AF1959).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1959
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1959-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

IL-12 R beta 2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Cys28-Asn622
Accession # Q99665
Specificity
Detects human and mouse IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL‑12 R beta 1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL12RB2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1959 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 2 Antibody

  • IL-12 R beta 2
  • IL-12 receptor beta 2
  • IL-12 receptor subunit beta-2
  • IL12R beta 2
  • IL-12R subunit beta-2
  • IL12RB2
  • IL-12Rb2
  • IL-12R-beta-2
  • interleukin 12 receptor, beta 2
  • interleukin-12 receptor beta-2 chain
  • interleukin-12 receptor subunit beta-2

Background

Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma . It is also required for the expansion and activation Th1 cells (1, 2).

The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL-12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) residue protein with a putative 27 aa residue signal peptide that is cleaved to generate the mature protein with a 595 aa residue extracellular domain, a 24 aa residue transmembrane domain and a 216 aa residue cytoplasmic region. Human and mouse IL-12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL-12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).

  1. Trinchieri, G. et al. (2003) Immunity 19:641. 
  2. Brombacher, F. et al. (2003) Trends in Immunol. 23:207. 
  3. Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
  4. Rogge, L. et al. (1997) J. Exp. Med. 185:825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(455 Publications)
Species: Hu
Applications: BA
IL-12 R beta 1
Antibody [Unconjugate
AF839
IL-12 R beta 1 Antibody [Unconjugated]
(3 Publications)
Species: Hu
Applications: WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
STAT4 [p Tyr693]
Antibody [Unconjugate ...
AF4319
Western Blot STAT4 [p Tyr693] Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
T-bet/TBX21
Antibody (525803) [Un ...
MAB5385
Western Blot T-bet/TBX21 Antibody (525803) [Unconjugated]
Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]
Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]
(4 Publications)
Species: Hu
Applications: ICC, ICFlow, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
IL-18/IL-1F4 [HRP]
7625
IL-18/IL-1F4 [HRP]
(54 Publications)
Species: Mu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-23R Antibody
NB600-1147
Simple Western IL-23R Antibody
Western Blot IL-23R Antibody
Western Blot IL-23R Antibody
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: Flow-CS, Flow, IHC, IHC-P, Simple Western, WB
IL-12
[Unconjugated]
419-ML
SDS-PAGE IL-12 [Unconjugated]
Bioactivity IL-12 [Unconjugated]
(273 Publications)
Species: Mu
Applications: BA
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
SEC-MALS IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
Annexin A6 Antibody
NBP1-90149
Western Blot Annexin A6 Antibody
Immunohistochemistry-Paraffin Annexin A6 Antibody
Immunohistochemistry-Paraffin Annexin A6 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA

UBASH3B/STS1/Tula-2 An
AF6696
Western Blot UBASH3B/STS1/Tula-2 Antibody
Immunocytochemistry UBASH3B/STS1/Tula-2 Antibody
Species: Hu, Mu, Rt
Applications: ICC, WB
IL-12/IL-23 p40
[Biotin]
DY499
N/A IL-12/IL-23 p40 [Biotin]
(51 Publications)
Species: Mu
Applications: ELISA
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
IL-5 [HRP]
M5000
N/A IL-5 [HRP]
N/A IL-5 [HRP]
(92 Publications)
Species: Mu
Applications: ELISA

Publications for IL-12 R beta 2 Antibody (AF1959)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


Filter By Application
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for IL-12 R beta 2 Antibody (AF1959) (0)

There are no reviews for IL-12 R beta 2 Antibody (AF1959). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-12 R beta 2 Antibody (AF1959) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-12 R beta 2 Products

Blogs on IL-12 R beta 2

There are no specific blogs for IL-12 R beta 2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-12 R beta 2 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol IL12RB2
Uniprot