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Canine IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
3.4 pg/mL

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Canine IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
3.4 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
80 - 95%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Canine IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A