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Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

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ELISA: IL-1 alpha/IL-1F1 ELISA Kit [Biotin] [NBP1-92699] - Standard curve

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
4.0 pg/mL

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Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary

Description
The rat IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-1alpha
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-1 alpha positive serum. There was no crossreactivity detected
Standard Curve Range
15.6 - 1,000 pg/mL
Sensitivity
4.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 5.0%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1alpha
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-rat IL-1alpha polyclonal antibody
  5. Rat IL-1alpha Standard lyophilized, 2 ng/ml upon reconstitution
  6. Stop Solution (1M Phosphoric acid)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A