Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary
Description |
The rat IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-1alpha |
Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-1 alpha positive serum. There was no crossreactivity detected |
Standard Curve Range |
15.6 - 1,000 pg/mL |
Sensitivity |
4.0 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 10% |
Intra-Assay |
CV% < 5.0% |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
IL1A |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1alpha
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-rat IL-1alpha polyclonal antibody
- Rat IL-1alpha Standard lyophilized, 2 ng/ml upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)
Background
The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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