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Goat anti-Mouse IgG Secondary Antibody [Fluorescein]

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HT1080 humanfibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha V/CD51Monoclonal Antibody (Catalog # MAB1219,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Fluorescein

Order Details

Goat anti-Mouse IgG Secondary Antibody [Fluorescein] Summary

Immunogen
Mouse IgG heavy and light chains
Specificity
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
F0103B in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [Fluorescein]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (F0103B)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-P, Secondary Antibody.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
IHC-P
(1)
Secondary Antibody
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using F0103B Applications Species
Liao R, Liu Q, Zheng Z, Fan J, Peng W, Kong Q, He H, Yang S, Chen W, Tang X, Yu X Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis. PLoS ONE, 2015;10(7):e0132724. 2015 [PMID: 26161538] (IHC-P, Mouse) IHC-P Mouse
Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014;14(0):118. 2014 [PMID: 24559071] (Flow Cytometry, Human) Flow Cytometry Human
Fan, Yuqi, Wang, Lianshen, Li, Yuanmin, Yin, Zhaofang, Xu, Zuojun, Wang, Changqia Quantification of endothelial microparticles on modified cytometric bead assay and prognosis in chest pain patients. Circ J, 2014;78(1):206-14. 2014 [PMID: 24200872] (Flow Cytometry, Human) Flow Cytometry Human
Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway. Biomaterials, 2009;30(17):3084-90. 2009 [PMID: 19250666] (ICC, Human) ICC Human
Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006;241(1):7-13. 2006 [PMID: 16914126] (Secondary Antibody, Human) Secondary Antibody Human

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Product General Protocols

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FAQs for IgG Antibody (F0103B) (0)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1