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AICDA Antibody (359218)

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AICDA was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Rat Anti-Human AICDA Monoclonal Antibody (Catalog # MAB39102) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
AICDA was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Rat Anti-Human AICDA Monoclonal Antibody (Catalog # MAB39102) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
359218
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB39102-SP
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AICDA Antibody (359218) Summary

Immunogen
E. coli-derived recombinant human AICDA
Met1-Leu198
Accession # Q9GZX7
Specificity
Detects human AICDA in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
AICDA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB39102 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AICDA Antibody (359218)

  • activation-induced cytidine deaminase
  • AICDA
  • AID
  • AIDCytidine aminohydrolase
  • Arp2
  • CDA2
  • HIGM2
  • HIGM2EC 3.5.4.5
  • integrated into Burkitt's lymphoma cell line Ramos

Background

AICDA, also known as AID, is a 24 kDa cytidine deaminase that converts deoxycytidine to deoxyuridine in single stranded DNA. Its expression is restricted to activated lymph node and tonsillar B cells and is required for terminal B cell differentiation. Loss of function mutations of AICDA cause severe humoral immunodeficiency by preventing immunoglobulin class switching and somatic hypermutation. Human AICDA shares 92% - 95% amino acid sequence identity with bovine, canine, and mouse AICDA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AICDA Antibody (MAB39102)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol AICDA
Uniprot