Novus Biologicals products are now on bio-techne.com

IgA2 Antibody (RM125)

Images

 
Immunohistochemistry-Paraffin: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - IgA2 stained human tonsil FFPE, 20X magnification. Antigen retrieval was performed with citrate pH 6 buffer. Primary antibody at 1:8000. ...read more
Immunohistochemistry: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Immunohistochemistry of Human Tonsil Tissue using NBP2-62025.
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - ELISA of human immunoglobulins shows NBP2-62025 only reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with ...read more
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - A titer ELISA using NBP2-62025. The plate was coated with different amounts of human IgA2. A serial dilution of NBP2-62025 was used as the primary antibody. An ...read more
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Sandwich ELISA using NBP2-62025 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody (NBP2-77396) as the detection ...read more
ELISA: IgA2 Antibody (RM125) [NBP2-62025] - A titer ELISA using Clone RM125. The plate was coated with different amounts of human IgA2. A serial dilution of Clone RM125 was used as the primary antibody. An alkaline ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF, IHC, ELISA
Clone
RM125
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

IgA2 Antibody (RM125) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human IgA2
Specificity
This antibody reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IGHA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 0.5ug/ml - 2ug/ml
  • Immunohistochemistry 0.1 - 1ug/ml
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA Capture 50 - 200ng/well
  • Sandwich ELISA Detection 0.05 - 0.2ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP2-62025 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IgA2 Antibody (RM125)

  • immunoglobulin heavy constant alpha 2 (A2m marker)

Background

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with human IgA2 from myeloma patient serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61794
Species: Hu, Pm, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1938
Species: Hu
Applications: ICC, IP, Simple Western, WB
NBP3-11412
Species: Sh
Applications: ELISA, IHC, IHC-Fr, WB
NBP2-62076
Species: Hu
Applications: ELISA, ELISA(Cap), ELISA(Det)
NB100-62667
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
NBP1-87466
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-37585
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-56654
Species: Hu
Applications: IHC, IHC-P, WB
H00003703-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, IP, WB
NBP2-79716
Species: Hu
Applications: IHC, IHC-P, PA
NBP3-17131
Species: Hu
Applications: IHC, IHC-P
NB7276
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NB7463
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
DY2037
Species: Hu
Applications: ELISA

Publications for IgA2 Antibody (NBP2-62025) (0)

There are no publications for IgA2 Antibody (NBP2-62025).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for IgA2 Antibody (NBP2-62025) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-62025:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgA2 NBP2-62025
Enlarge
5
reviewed by:
Christopher Nazaroff
IHC-P Human 11/27/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsil tissue
SpeciesHuman
LotR-09-01750

Comments

CommentsAntigen retrieval was done with citrate pH6 for 15 mins at 100C and allowed to cool 30 minutes at room temperature. Primary was diluted 1:8000 and incubated at room temperature for 30 mins in Dako antibody diluent (S3022). Secondary ImmPRESS goat anti-rabbit HRP polymer was incubate 30 minutes at room temperature and signal was developed with SIgnalStain DAB chromagen.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgA2 Antibody (NBP2-62025) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IgA2 Products

Array NBP2-62025

Research Areas for IgA2 Antibody (NBP2-62025)

Find related products by research area.

Blogs on IgA2

There are no specific blogs for IgA2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Christopher Nazaroff
11/27/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IGHA2