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Mouse IGFBP-1 ELISA Kit (Colorimetric)

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ELISA: Mouse IGFBP-1 ELISA Kit (Colorimetric) [NBP2-80238] - Samples were spiked with high concentrations of Mouse IGFBP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse IGFBP-1 ELISA Kit (Colorimetric) [NBP2-80238] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL

Order Details

Mouse IGFBP-1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse IGFBP-1 in samples. No significant cross-reactivity or interference between Mouse IGFBP-1 and analogues was observed.
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.16%
Intra-Assay
CV% < 5.05%
Spike Recovery
87-107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IGFBP1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse IGFBP-1 ELISA Kit (Colorimetric)

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Background

IGFBP1 is a gene that codes for an IGF-binding protein that circulates in the plasma and prolongs the half-life of IGFs and works to either inhibit of stimulate the growth promoting effects while also promoting cell migration and has a length of 259 amino acids and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including protein-energy malnutrition, pre-eclampsia, persistent fetal circulation syndrome, Silver-Russell syndrome, growth hormone deficiency, Ehlers-Danlos syndrome, glucose intolerance, Prader-Willi syndrome, and chronic fatigue syndrome. IGFBP1 has also been shown to have interactions with IGF1, IGF2, MMP26, TF, and ARNT in pathways such as the myometrial relaxation and contraction, HIF-1-alpha transcription factor, IGF regulation, and protein metabolism pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on IGFBP-1.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

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Bioinformatics

Gene Symbol IGFBP1
Uniprot