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IGF-I/IGF-1 Antibody

 (10 Publications)
 (1 Review)
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Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody [NBP2-16929] - SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody [NBP2-16929] - SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at ...read more
Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Gp, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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IGF-I/IGF-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IGF-I/IGF-1. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Rat (94%), Porcine (96%), Bovine (97%), Guinea Pig (100%), Rhesus Macaque (100%), Canine (98%), Sheep (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16929 in the following applications:
Publications
Read Publications using
NBP2-16929 in the following applications:

  • IF/IHC
    1 publication
  • IHC
    1 publication
  • WB
    3 publications

Reactivity Notes

Armadillo (100%), Xenopus laevis (80%), Rabbit (82%), Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for IGF-I/IGF-1 Antibody

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IGF-I/IGF-1 Antibody (NBP2-16929)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IF/IHC, IHC, WB.


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IF/IHC
(1)
IHC
(1)
WB
(3)
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(1)
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(1)
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Showing Publications 1 - 10 of 10.
Publications using NBP2-16929 Applications Species
Suzuki T, Scala F, Demetrius DL et al. IL-15 Prolongs Anagen, Stimulates Proliferation, and Suppresses Apoptosis in the Hair Matrix of Human Scalp Hair Follicles The Journal of investigative dermatology 2023-08-04 [PMID: 37543243]
Shamardl HAMA, Ibrahim NA, Merzeban DH et al. Resveratrol and Dulaglutide ameliorate adiposity and liver dysfunction in rats with diet-induced metabolic syndrome: Role of SIRT-1 / adipokines / PPAR? and IGF-1 Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 2023-03-29 [PMID: 36991247] (IHC, Rat) IHC Rat
Ladraa S, Zerbib L, Bayard C et al. PIK3CA gain-of-function mutation in adipose tissue induces metabolic reprogramming with Warburg-like effect and severe endocrine disruption Science advances 2022-12-09 [PMID: 36490341]
Jia ZQ, Zuo C, Yue WF Kurarinone alleviates hemin-induced neuroinflammation and microglia-mediated neurotoxicity by shifting microglial M1/M2 polarization via regulating the IGF1/PI3K/Akt signaling The Kaohsiung journal of medical sciences 2022-09-28 [PMID: 36169245] (WB, Human)

Details:
Dilution used in WB 1:1000 		WB Human
Luo M, Zeng Q, Jiang K et al. Estrogen deficiency exacerbates learning and memory deficits associated with glucose metabolism disorder in APP/PS1 double transgenic female mice Genes & Diseases 2022-09-01 [PMID: 35873026] (WB) WB
Yan X, Managlia E, Zhao YY et al. Macrophage-derived IGF-1 protects the neonatal intestine against necrotizing enterocolitis by promoting microvascular development Communications biology 2022-04-06 [PMID: 35388142] (WB, Mouse) WB Mouse
MaihOfer J, Madry H, Rey-Rico A et al. Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo Advanced materials (Deerfield Beach, Fla.) 2021-03-18 [PMID: 33734514] (IF/IHC) IF/IHC
Shymaa M. El-Komy, Ayman A. Saleh, Tamer M. Abdel-Hamid, Mohammed A. El-Magd Association of GHR Polymorphisms with Milk Production in Buffaloes Animals : an Open Access Journal from MDPI 2020-07-15 [PMID: 32679878]
Song J, Hao L, Wei W et al. A SNP in the 3'UTR of porcine IGF-1 gene interacts with miR-new14 to affect IGF-1 expression, proliferation and apoptosis of PK-15 cell Domest Anim Endocrinol 2020-03-15 [PMID: 32171113]
Lin CW Jan MS Kuo JH. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. [PMID: 26158199]

Review for IGF-I/IGF-1 Antibody (NBP2-16929) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-16929:
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Images Ratings Applications Species Date Details
Western Blot IGF-I/IGF-1 NBP2-16929
Enlarge 		4
reviewed by:
Andrew Cosgrove
 WB Mouse 12/16/2013
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Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
Lot40807

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Western Blot IGF-I/IGF-1 NBP2-16929
Enlarge
 Andrew Cosgrove
12/16/2013
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol IGF1