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IFN-gamma R1/CD119 Antibody [Unconjugated]

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Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). ...read more
IFN-gamma R1/CD119 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IFN-gamma R1/CD119 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1/CD119
Glu18-Gly245
Accession # P15260
Specificity
Detects IFN‑ gamma  R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
IFN-gamma  R2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFNGR1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.5-2 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF673 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R1/CD119 Antibody [Unconjugated]

  • AVP, type 2
  • CD119 antigen
  • CD119
  • FLJ45734
  • IFN-gamma R1
  • IFN-gamma receptor 1
  • IFNgammaR1
  • IFN-gamma-R1
  • IFNGR
  • IFNGR1
  • IFN-gR1
  • immune interferon receptor 1
  • interferon gamma receptor 1
  • interferon-gamma receptor alpha chain
  • type 2

Background

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma  R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFN-gamma R1/CD119 Antibody (AF673)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Capture, Flow Cytometry, IHC, IHC-P, Neutralization.


Filter By Application
ELISA Capture
(1)
Flow Cytometry
(1)
IHC
(1)
IHC-P
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF673 Applications Species
RV Broad, SJ Jones, MC Teske, LM Wastall, AM Hanby, JL Thorne, TA Hughes Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy British Journal of Cancer, 2021-01-04;0(0):. 2021-01-04 [PMID: 33398063] (Flow Cytometry, IHC, Human) Flow Cytometry, IHC Human
Qin Hao, Hua Tang Interferon-gamma and Smac mimetics synergize to induce apoptosis of lung cancer cells in a TNF alpha -independent manner Cancer Cell International 2018-12-01 [PMID: 29946223]
SY Zhang, NE Clark, CA Freije, E Pauwels, AJ Taggart, S Okada, H Mandel, P Garcia, MJ Ciancanell, A Biran, FG Lafaille, M Tsumura, A Cobat, J Luo, S Volpi, B Zimmer, S Sakata, A Dinis, O Ohara, EJ Garcia Rei, K Dobbs, M Hasek, SP Holloway, K McCammon, SA Hussong, N DeRosa, CE Van Skike, A Katolik, L Lorenzo, M Hyodo, E Faria, R Halwani, R Fukuhara, GA Smith, V Galvan, MJ Damha, S Al-Muhsen, Y Itan, JD Boeke, LD Notarangel, L Studer, M Kobayashi, L Diogo, WG Fairbrothe, L Abel, BR Rosenberg, PJ Hart, A Etzioni, JL Casanova Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection Cell, 2018-02-22;172(5):952-965.e18. 2018-02-22 [PMID: 29474921] (ELISA Capture, Human) ELISA Capture Human
Allison B. Reiss, Kamran Anwar, Joan T. Merrill, Edwin S. L. Chan, Nahel W. Awadallah, Bruce N. Cronstein et al. Plasma from systemic lupus patients compromises cholesterol homeostasis: a potential mechanism linking autoimmunity to atherosclerotic cardiovascular disease Rheumatology International 2010-03-01 [PMID: 19547978]
Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Neutralization, Human) Neutralization Human
Saidi RF, Williams F, Silberberg B, Mittal VK, ReMine SG, Jacobs MJ Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor. Surgery, 2006-06-01;139(6):743-8. 2006-06-01 [PMID: 16782428] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol IFNGR1
Uniprot