Home » IFN-gamma R1/CD119 » IFN-gamma R1/CD119 Antibodies » IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]
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IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]

 (13 Publications)
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Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Anti-Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma R1/CD119 Monoclonal Antibody (Catalog # MAB6731). The ND50 is typically 0.01-0.03 µg/mL.
Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
92101
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6731U-100UG
Alexa Fluor 405
FAB6731V-100UG
Alexa Fluor 488
FAB6731G-100UG
Alexa Fluor 532
FAB6731X
Alexa Fluor 594
FAB6731T-100UG
Alexa Fluor 647
FAB6731R-100UG
Alexa Fluor 700
FAB6731N-100UG
Alexa Fluor 750
FAB6731S-100UG
Allophycocyanin
FAB6731A
Allophycocyanin/Cy7
FAB6731APCCY7
Biotin
FAB6731B
DyLight 350
FAB6731D
DyLight 405
FAB6731E
DyLight 488
FAB6731K
DyLight 550
FAB6731L
DyLight 594
FAB6731M
DyLight 650
FAB6731W
DyLight 680
FAB6731Y
DyLight 755
FAB6731Z
Fluorescein
FAB673F
HRP
FAB6731H
Janelia Fluor 525
FAB6731JF525
Janelia Fluor 549
FAB6731I
Janelia Fluor 646
FAB6731J
Janelia Fluor 669
FAB6731JF669
PE/Atto594
FAB6731PEATT594
PE/Cy5.5
FAB6731PECY55
PE/Cy7
FAB6731PECY7
PerCP
FAB6731C
Phycoerythrin
FAB673P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB673F
Carrier Free
FAB673P
Carrier Free
MAB6731-SP
Carrier Free
FAB6731V-100UG
Carrier Free
FAB6731U-100UG
Carrier Free
FAB6731T-100UG
Carrier Free
FAB6731S-100UG
Carrier Free
FAB6731R-100UG
Carrier Free
FAB6731N-100UG
Carrier Free
FAB6731G-100UG
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IFN-gamma R1/CD119 Antibody (92101) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1
Glu18-Gly245
Accession # P15260.1
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFNGR1
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.01-0.03 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB6731 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]

  • AVP, type 2
  • CD119 antigen
  • CD119
  • FLJ45734
  • IFN-gamma R1
  • IFN-gamma receptor 1
  • IFNgammaR1
  • IFN-gamma-R1
  • IFNGR
  • IFNGR1
  • IFN-gR1
  • immune interferon receptor 1
  • interferon gamma receptor 1
  • interferon-gamma receptor alpha chain
  • type 2

Background

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]
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Publications for IFN-gamma R1/CD119 Antibody (MAB6731)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, Neutralization, Neutralizing, Western Blot.


Filter By Application
Flow Cytometry
(2)
Neutralization
(9)
Neutralizing
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(12)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB6731 Applications Species
Menevse, AN;Ammer, LM;Vollmann-Zwerenz, A;Kupczyk, M;Lorenz, J;Weidner, L;Hussein, A;Sax, J;M�hlbauer, J;Heuschneider, N;Rohrmus, C;Mai, LS;Jachnik, B;Stamova, S;Volpin, V;Durst, FC;Sorrentino, A;Xydia, M;Milenkovic, VM;Bader, S;Braun, FK;Wetzel, C;Albert, NL;Tonn, JC;Bartenstein, P;Proescholdt, M;Schmidt, NO;Linker, RA;Riemenschneider, MJ;Beckhove, P;Hau, P; TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastoma Acta neuropathologica communications 2023-05-08 [PMID: 37158962] (Neutralization, Human) Neutralization Human
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-03-25;13(1):1606. 2022-03-25 [PMID: 35338135] (Western Blot, Human) Western Blot Human
S Mairpady S, A Gómez-Meji, TA Schweizer, M Huemer, CC Chang, C Acevedo, J Bergada-Pi, C Vulin, DA Hofmaenner, TC Scheier, S Hertegonne, E Parietti, N Miroshniko, PD Wendel Gar, MP Hilty, PK Buehler, RA Schuepbach, SD Brugger, AS Zinkernage Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-19 PloS Pathogens, 2022-01-10;18(1):e1010176. 2022-01-10 [PMID: 35007290] (Neutralization, Human) Neutralization Human
P Voabil, M de Bruijn, LM Roelofsen, SH Hendriks, S Brokamp, M van den Br, A Broeks, J Sanders, P Herzig, A Zippelius, CU Blank, KJ Hartemink, K Monkhorst, JBAG Haanen, TN Schumacher, DS Thommen An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer Nature Medicine, 2021-07-08;27(7):1250-1261. 2021-07-08 [PMID: 34239134] (Neutralization, Human) Neutralization Human
Yan-Fang Xing, Jia-Rong Cai, Jun-Jian Qin, Wen-Ying Zhou, Can-Ming Li, Xing Li Expansion of Monocytic Myeloid-Derived Suppressor Cells in Patients Under Hemodialysis Might Lead to Cardiovascular and Cerebrovascular Events Frontiers in Immunology 2021-01-29 [PMID: 33584644]
PA Szabo, HM Levitin, M Miron, ME Snyder, T Senda, J Yuan, YL Cheng, EC Bush, P Dogra, P Thapa, DL Farber, PA Sims Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease Nat Commun, 2019-10-17;10(1):4706. 2019-10-17 [PMID: 31624246] (Neutralization, Human) Neutralization Human
L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum J. Allergy Clin. Immunol., 2018-01-31;0(0):. 2018-01-31 [PMID: 29371118] (Neutralizing, Human) Neutralizing Human
C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus J. Virol., 2018-03-14;0(0):. 2018-03-14 [PMID: 29343571] (Flow Cytometry, Human) Flow Cytometry Human
SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing PLoS ONE, 2017-08-24;12(8):e0183390. 2017-08-24 [PMID: 28837681] (Neutralization, Human) Neutralization Human
LM Feldhoff, CM Rueda, ME Moreno-Fer, J Sauer, CM Jackson, CA Chougnet, J Rupp IL-1? induced HIF-1? inhibits the differentiation of human FOXP3(+) T cells Sci Rep, 2017-03-28;7(1):465. 2017-03-28 [PMID: 28352109] (Neutralization, Human) Neutralization Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol IFNGR1
Uniprot