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IFN-beta Antibody [Unconjugated]

 (11 Publications)
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Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND50 is typically 0.05-0.2 µg/mL.
Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF814
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF814-SP
100% Guarantee on every product

IFN-beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IFN-beta
Specificity
Detects human IFN-beta in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFNB1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Neutralization 0.100 - 1.00 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF814 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-beta Antibody [Unconjugated]

  • Fibroblast interferon
  • IFB
  • IFBIFNB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFN-beta Antibody (AF814)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


Filter By Application
Neutralization
(7)
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Human
(7)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF814 Applications Species
LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC Data in Brief, 2022-11-11;45(0):108743. 2022-11-11 [PMID: 36426057] (Neutralization, Human) Neutralization Human
J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer Cancer Research, 2022-12-16;0(0):. 12/16/2022 [PMID: 36222718]
D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-11-11;0(0):e12924. 2021-11-11 [PMID: 34762341] (Neutralization, Human) Neutralization Human
Sultan Doğanay, Maurice Youzong Lee, Alina Baum, Jessie Peh, Sun-Young Hwang, Joo-Yeon Yoo et al. Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic IFNB1 expression Integrative Biology 11/13/2017 [PMID: 29098213]
Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Kruger DH, Klempa B Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE, 2012-04-24;7(4):e35587. 2012-04-24 [PMID: 22545121] (Neutralization, Human) Neutralization Human
Boris Klempa, Peter T. Witkowski, Elena Popugaeva, Brita Auste, Lamine Koivogui, Elisabeth Fichet-Calvet et al. Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses Journal of Virology 4/1/2012 [PMID: 22278233]
Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. PLoS Pathog., 2011-04-14;7(4):e1002016. 2011-04-14 [PMID: 21533215] (Neutralization, Human) Neutralization Human
Joseph Prescott, Pamela Hall, Mariana Acuna-Retamar, Chunyan Ye, Marc G. Wathelet, Hideki Ebihara et al. New World hantaviruses activate IFNlambda production in type I IFN-deficient vero E6 cells PLoS ONE 6/17/2010 [PMID: 20567522]
Rascle A, Neumann T, Raschta AS, Neumann A, Heining E, Kastner J, Witzgall R The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes. Exp. Cell Res., 2008-10-28;315(1):76-96. 2008-10-28 [PMID: 18996370] (Neutralization, Human) Neutralization Human
Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T TLR3 can directly trigger apoptosis in human cancer cells. J. Immunol., 2006-04-15;176(8):4894-901. 2006-04-15 [PMID: 16585585] (Neutralization, Human) Neutralization Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol IFNB1