Home » IFN-beta » IFN-beta Antibodies » IFN-beta Antibody (76703) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

IFN-beta Antibody (76703) [Unconjugated]

 (7 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ beta Monoclonal Antibody (Catalog # MAB814). The ND50 is typically 7-21 µg/mL.
Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as ...read more
M1 macrophages treated with 50 ng/mL each of rhGM-CSF (215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (285-IF) and restimulated with monensin for 3 hours (3uM) were stained with Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N
Clone
76703
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB814-SP
Carrier Free
MAB814-500
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

IFN-beta Antibody (76703) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IFN-beta
Met22-Asn187
Accession # P01574
Specificity
Detects human IFN-beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IFN‑ alpha .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFNB1
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 uL/10^6 cells
  • Neutralization 1.50-18.0 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB814 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-beta Antibody (76703) [Unconjugated]

  • Fibroblast interferon
  • IFB
  • IFBIFNB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IFN-alpha 1
Antibody
NBP2-75930
Western Blot IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(453 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA
TLR3 Antibody
(40C1285.6) - BSA Fre
NBP2-24875
Flow Cytometry TLR3 Antibody (40C1285.6) - BSA Free
Western Blot TLR3 Antibody (40C1285.6) - BSA Free
Flow Cytometry TLR3 Antibody (40C1285.6) - BSA Free
(87 Publications)
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, WB
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(141 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
TRIF/TICAM1
Antibody - BSA Free
NB120-13810
Knockdown Validated TRIF/TICAM1 Antibody - BSA Free
Unknown TRIF/TICAM1 Antibody - BSA Free
Immunohistochemistry-Paraffin TRIF/TICAM1 Antibody - BSA Free
(37 Publications)
Species: Mu, Po, Rt
Applications: IB, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
IRF3 Antibody
(SD2062)
NBP2-67741
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunohistochemistry-Paraffin IRF3 Antibody (SD2062)
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
STAT1 [p Tyr701]
Antibody [Unconjugate ...
AF2894
Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]
Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
IRF1 Antibody
[Unconjugated]
AF4715
Western Blot IRF1 Antibody [Unconjugated]
Western Blot IRF1 Antibody [Unconjugated]
(1 Review)
(2 Publications)
Species: Mu
Applications: WB
IL-1 beta/IL-1F2
[Unconjugated]
201-LB
SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]
Bioactivity IL-1 beta/IL-1F2 [Unconjugated]
SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]
(594 Publications)
Species: Hu
Applications: BA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
RIG-I Antibody
AF4859
Western Blot RIG-I Antibody
(2 Publications)
Species: Hu
Applications: WB
MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
(3 Reviews)
(99 Publications)
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
TLR2 Antibody
(TL2.1) - BSA Free
NB100-56722
Flow (Cell Surface) TLR2 Antibody (TL2.1) - BSA Free
Flow Cytometry TLR2 Antibody (TL2.1) - BSA Free
Block/ Neutralize TLR2 Antibody (TL2.1) - BSA Free
(64 Publications)
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
MAVS Antibody -
BSA Free
NB100-80859
Immunocytochemistry/ Immunofluorescence MAVS Antibody - BSA Free
Western Blot MAVS Antibody - BSA Free
Immunohistochemistry-Paraffin MAVS Antibody - BSA Free
(1 Review)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
IFN-beta Antibody
(76703) [Unconjugated ...
MAB814
IFN-beta Antibody (76703) [Unconjugated]
Species: Hu
Applications: WB, Flow, B/N

Publications for IFN-beta Antibody (MAB814)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB814 Applications Species
Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer Cancer Immunology Research 7/1/2022 [PMID: 35561311]
Wuchang Zhang, Wei Liu, Lingfei Jia, Demeng Chen, Insoon Chang, Michael Lake et al. Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress Molecular Cell 2021-05-01 [PMID: 33743195]
Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al. Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro International Journal of Medical Sciences 2020-02-18 [PMID: 32210710]
Y Liu, L Qu, Y Liu, B Roizman, GG Zhou PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2 Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):. 2017-07-31 [PMID: 28760986] (Neutralization, Human) Neutralization Human
Gupta S, Termini J, Issac B, Guirado E, Stone G Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors. PLoS ONE, 2016-02-05;11(2):e0148929. 2016-02-05 [PMID: 26849062] (Neutralization, Human) Neutralization Human
Huizinga R, van Rijs W, Bajramovic J, Kuijf M, Laman J, Samsom J, Jacobs B Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-beta and TNF-alpha. J Immunol, 2013-10-28;191(11):5636-45. 2013-10-28 [PMID: 24166974] (Neutralization, Human) Neutralization Human
Meager A, Dolman C, Dilger P, Bird C, Giovannoni G, Schellekens H, Thorpe R, Wadhwa M An assessment of biological potency and molecular characteristics of different innovator and noninnovator interferon-beta products. J. Interferon Cytokine Res., 2010-12-07;31(4):383-92. 2010-12-07 [PMID: 21138379] (Western Blot, Human) Western Blot Human

Reviews for IFN-beta Antibody (MAB814) (0)

There are no reviews for IFN-beta Antibody (MAB814). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFN-beta Antibody (MAB814) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IFN-beta Products

Blogs on IFN-beta.

Check out the latest blog posts on IFN-beta.
STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IFN-beta Antibody (76703) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol IFNB1
Uniprot