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ICA1 Antibody

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Western Blot: ICA1 Antibody [NBP2-16909] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: ICA1 Antibody [NBP2-16909] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: ICA1 Antibody [NBP2-16909] - Immunohistochemical analysis of paraffin-embedded A2780-CP70 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ICA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ICA1. The exact sequence is proprietary.
Localization
Cytoplasm; cytosol; Golgi apparatus membrane; Peripheral membrane protein (Potential); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein (Potential); secretory vesicle; synaptic vesicle membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ICA1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ICA1 Antibody

  • diabetes mellitus type I autoantigen
  • ICA1
  • ICA69
  • ICAp69
  • ICAp69Islet cell autoantigen p69,69 kDa islet cell autoantigen
  • islet cell autoantigen 1 (69kD)
  • islet cell autoantigen 1 isoform
  • islet cell autoantigen 1
  • islet cell autoantigen 1, 69kDa
  • p69

Background

This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol ICA1
Uniprot