Home » IL-17/IL-17A » IL-17/IL-17A ELISA Kits » Human IL-17 Quantikine HS ELISA Kit
Novus Biologicals products are now on bio-techne.com

Human IL-17 Quantikine HS ELISA Kit

 (10 Publications)
 See All Related
Submit a Review

Images

 

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human IL-17 Quantikine HS ELISA Kit Summary

Background
The Quantikine® HS Human IL-17 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure IL-17 levels in serum and plasma. It contains HEK293-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to... the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.
Show More
Specificity
Natural and recombinant human IL-17
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using HS170.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-17 Quantikine HS ELISA Kit

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 
A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha , IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

Customers Who Viewed This Item Also Viewed...

IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(660 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(301 Publications)
Species: Mu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IL-22
[Unconjugated]
782-IL/CF
SDS-PAGE IL-22 [Unconjugated]
Bioactivity IL-22 [Unconjugated]
(65 Publications)
Species: Hu
Applications: BA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(451 Publications)
Species: Hu
Applications: BA
IL-17D Antibody
(246018) [Unconjugate
MAB15041
Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]
Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
FoxP3 Antibody -
BSA Free
NB100-39002
Flow Cytometry FoxP3 Antibody - BSA Free
Flow Cytometry FoxP3 Antibody - BSA Free
Immunohistochemistry-Paraffin FoxP3 Antibody - BSA Free
(2 Reviews)
(62 Publications)
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
IL-17F [Biotin]
DY1335B
N/A IL-17F [Biotin]
N/A IL-17F [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
IL-37/IL-1F7
[Biotin]
DY1975
N/A IL-37/IL-1F7 [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]
(148 Publications)
Species: Mu
Applications: ELISA
IL-21 Antibody
[Unconjugated]
AF594
Immunocytochemistry IL-21 Antibody [Unconjugated]
Neutralization IL-21 Antibody [Unconjugated]
(80 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
IL-5 [HRP]
M5000
N/A IL-5 [HRP]
N/A IL-5 [HRP]
(92 Publications)
Species: Mu
Applications: ELISA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(255 Publications)
Species: Hu
Applications: ELISA
IL-17RA/IL-17R
Antibody (133617) [Un ...
MAB177
Neutralization IL-17RA/IL-17R Antibody (133617) [Unconjugated]
Flow Cytometry IL-17RA/IL-17R Antibody (133617) [Unconjugated]
Knockout Validated IL-17RA/IL-17R Antibody (133617) [Unconjugated]
(20 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, KO, Neut, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Human IL-17
Quantikine HS ELISA K
HS170
Human IL-17 Quantikine HS ELISA Kit
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-17/IL-17A (HS170)(10)

We have publications tested in 2 confirmed species: Human, Rat.


Filter By Application
All Applications
Filter By Species
Human
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using HS170 Applications Species
Pletikosic, I;Marasovic Krstulovic, D;Bakovic, D;Susilovic Grabovac, Z;Tandara, L;Martinovic Kaliterna, D; Association of inflammatory biomarkers and disease activity with subclinical myocardial dysfunction in psoriatic arthritis Scientific reports 2023-06-26 [PMID: 37365233] (Human) Human
H Mostafavi, K Tharmaraja, J Vider, NP West, JR Freitas, B Cameron, PS Foster, LP Hueston, AR Lloyd, S Mahalingam, A Zaid Interleukin-17 contributes to Ross River virus-induced arthritis and myositis PloS Pathogens, 2022-02-10;18(2):e1010185. 2022-02-10 [PMID: 35143591] (Human) Human
A Prtina, N Rašeta Sim, T Milivojac, M Vujni?, M Grabež, D Djuric, MP Stojiljkov, V Soldat Sta, MJ ?oli?, R Škrbi? The Effect of Three-Month Vitamin D Supplementation on the Levels of Homocysteine Metabolism Markers and Inflammatory Cytokines in Sera of Psoriatic Patients Biomolecules, 2021-12-11;11(12):. 2021-12-11 [PMID: 34944509] (Human) Human
E Jahaj, AG Vassiliou, C Keskinidou, P Gallos, CS Vrettou, S Tsipilis, Z Mastora, SE Orfanos, I Dimopoulou, A Kotanidou Evaluating the Role of the Interleukin-23/17 Axis in Critically Ill COVID-19 Patients Journal of personalized medicine, 2021-09-07;11(9):. 2021-09-07 [PMID: 34575667] (Human) Human
LW Dong, XN Sun, ZC Ma, J Fu, FJ Liu, BL Huang, DC Liang, DM Sun, C Lan Increased Vdelta1gammadeltaT cells predominantly contributed to IL-17 production in the development of adult human post-infectious irritable bowel syndrome BMC gastroenterology, 2021-06-30;21(1):271. 2021-06-30 [PMID: 34193069] (Human) Human
MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri Immune Profiling To Predict Outcome of Clostridioides difficile Infection MBio, 2020-05-26;11(3):. 2020-05-26 [PMID: 32457246] (Human) Human
F Hernández-, MJ Ruiz-de-Le, I Rosado-Sán, E Vázquez, M Leal, S Moreno, F Vidal, J Blanco, YM Pacheco, A Vallejo New signatures of poor CD4 cell recovery after suppressive antiretroviral therapy in HIV-1-infected individuals: involvement of miR-192, IL-6, sCD14 and miR-144 Sci Rep, 2020-02-19;10(1):2937. 2020-02-19 [PMID: 32076107] (Human) Human
HR Ghaiad, AN Elmazny, MM Nooh, MM El-Sawalhi, AA Shaheen Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability J Adv Res, 2019-11-04;21(0):141-150. 2019-11-04 [PMID: 32071782] (Human) Human
J Yang, J Wang, X Liang, H Zhao, J Lu, Q Ma, B Jing, F Tian IL?1beta increases the expression of inflammatory factors in synovial fluid?derived fibroblast?like synoviocytes via activation of the NF?kappaB?mediated ERK?STAT1 signaling pathway Mol Med Rep, 2019-10-21;0(0):. 2019-10-21 [PMID: 31638264] (Rat) Rat
Joanna Domagala-K Elevated regulatory T cells, surface and intracellular CTLA-4 expression and interleukin-17 in the lung cancer microenvironment in humans Cancer Immunol. Immunother., 2016-11-19;0(0):. 2016-11-19 [PMID: 27866241] (Human) Human

Reviews for IL-17/IL-17A (HS170) (0)

There are no reviews for IL-17/IL-17A (HS170). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-17/IL-17A (HS170). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Additional IL-17/IL-17A Products

Blogs on IL-17/IL-17A

There are no specific blogs for IL-17/IL-17A, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human IL-17 Quantikine HS ELISA Kit and receive a gift card or discount.