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HTRA2/Omi Antibody (4H8C4)

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Western Blot: HTRA2/Omi Antibody (4H8C4) [NBP3-16294] - Western blot analysis of extracts of various cell lines, using HTRA2/Omi Rabbit mAb (NBP3-16294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: HTRA2/Omi Antibody (4H8C4) [NBP3-16294] - Western blot analysis of extracts of various cell lines, using HTRA2/Omi Rabbit mAb (NBP3-16294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
4H8C4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HTRA2/Omi Antibody (4H8C4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HTRA2/Omi (O43464). GTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVV
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HTRA2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for HTRA2/Omi Antibody (4H8C4)

  • High temperature requirement protein A2
  • HtrA serine peptidase 2
  • HTRA2
  • HtrA-like serine protease
  • Omi
  • OMIOmi stress-regulated endoprotease
  • PARK13EC 3.4.21.108
  • PRSS25
  • PRSS25serine, 25
  • Serine protease 25
  • serine protease HTRA2, mitochondrial
  • Serine proteinase OMI

Background

HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol HTRA2