Endo G Antibody - BSA Free

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 3 of 3.

Publications using NBP1-76657	Applications	Species
Wang W, Tan J, Liu X et al. Cytoplasmic Endonuclease G promotes nonalcoholic fatty liver disease via mTORC2-AKT-ACLY and endoplasmic reticulum stress Nature communications 2023-10-04 [PMID: 37794041] (ICC/IF, Human) Details: Dilution 1:100	ICC/IF	Human
Wang W, Li J, Tan J, et al. Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response Nature communications 2021-01-20 [PMID: 33473107] (ICC/IF, Human)	ICC/IF	Human
Chiu YS, Cheng YH, Lin SW et al. Bupivacaine induces apoptosis through caspase-dependent and -independent pathways in canine mammary tumor cells Res. Vet. Sci. 2015-03-25 [PMID: 25843897] (WB, Canine) Details: Endo G antibody was used at 1:1000dilution in WB assay for analysis of lysates from canine breast cancer DTK-SME cell line treated or not with Bupivacaine ( anesthetic for prolonged, local and regional anesthesia) for various time frames (0-48 hours; see protocol details in full text). Fig 3B shows the WB data and it is evident that Endo G is released from mitochondria of DTK-SME cells after 24 h of bupivacaine treatment.	WB	Canine

Array NBP1-76657

• Endo G Antibodies
• Endo G ELISA Kits
• Endo G Lysate
• Endo G Proteins