![Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Western-Blot-NB120-5479-img0016.jpg "Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | 2E1/53 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Human placental HSP60. |
| Epitope | Epitope mapping studies using human Hsp 60 deletion mutants suggest that this antibody binds between amino acids 211-288. |
| Localization | Mitochondrial/chloroplast |
| Marker | Mitochondria Marker |
| Specificity | Detects Hsp 60 from human cells, tissues and recombinant protein preparations. This displays no other protein or species cross-reactivity. |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HSPD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects an approx. 58 kDa protein. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS. |
| Publication using NB120-5479 | Applications | Species |
|---|---|---|
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
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Customer Experience using HSP60 Antibody I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat ... Read full blog post. |
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Heat Shock Proteins: An Overview Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis... Read full blog post. |
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The Heat is On: Heat Shock Proteins and the Link to Cancer Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0013.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.")
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0015.jpg "Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0004.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0007.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0008.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Heat Shock Protein 60 monoclonal antibody (2E1/53) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0010.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0011.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunocytochemistry-Immunofluorescence-NB120-5479-img0012.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 60 (Hsp60) monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunohistochemistry-Paraffin-NB120-5479-img0014.jpg "Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.")
![Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight rotating at 4C, washed in TBST, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP60-Antibody-2E1-53-Immunoprecipitation-NB120-5479-img0006.jpg "Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a HSP60 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody at a concentration of 1ug/ml overnight rotating at 4C, washed in TBST, and probed with a goat anti-mouse IgM secondary antibody at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection performed using SuperSignal West Dura.")
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