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HSP60 Antibody (2E1/53)

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Western Blot: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50ug of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells.
Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Anlysis of Hela Cells at a dilution of 1:200
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E1/53) [NB120-5479] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock ...read more
Immunohistochemistry-Paraffin: HSP60 Antibody (2E1/53) [NB120-5479] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues.
Immunoprecipitation: HSP60 Antibody (2E1/53) [NB120-5479] - Analysis of Heat Shock Protein 60 (HSP60) was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
2E1/53
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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HSP60 Antibody (2E1/53) Summary

Immunogen
Human placental HSP60.
Epitope
Epitope mapping studies using human Hsp 60 deletion mutants suggest that this antibody binds between amino acids 211-288.
Localization
Mitochondrial/chloroplast
Marker
Mitochondria Marker
Specificity
Detects Hsp 60 from human cells, tissues and recombinant protein preparations. This displays no other protein or species cross-reactivity.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
HSPD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1 - 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1 - 10 ug/ml
  • Immunoprecipitation 2 ug
  • Western Blot 1 - 10 ug/ml
Application Notes
WB: Detects an approx. 58 kDa protein.
Control
HSP60 Overexpression Lysate
Publications
Read Publication using NB120-5479.

Reactivity Notes

Cross-reactivity with Mouse, Rat and Monkey.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS.

Alternate Names for HSP60 Antibody (2E1/53)

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP60 Antibody (NB120-5479)(1)


Showing Publication 1 - 1 of 1.
Publication using NB120-5479 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP60 Antibody (NB120-5479) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional HSP60 Products

Research Areas for HSP60 Antibody (NB120-5479)

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Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat ...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol HSPD1
Entrez