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Hsp47 Antibody (M16.10A1) [Janelia Fluor® 549]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Pm, Rb, ShSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, KD
Clone
M16.10A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97491AF350
Alexa Fluor 405
NBP1-97491AF405
Alexa Fluor 488
NBP1-97491AF488
Alexa Fluor 532
NBP1-97491AF532
Alexa Fluor 594
NBP1-97491AF594
Alexa Fluor 647
NBP1-97491AF647
Alexa Fluor 700
NBP1-97491AF700
Alexa Fluor 750
NBP1-97491AF750
Allophycocyanin
NBP1-97491APC
Biotin
NBP1-97491B
DyLight 350
NBP1-97491UV
DyLight 405
NBP1-97491V
DyLight 488
NBP1-97491G
DyLight 550
NBP1-97491R
DyLight 594
NBP1-97491DL594
DyLight 650
NBP1-97491C
DyLight 680
NBP1-97491FR
DyLight 755
NBP1-97491IR
FITC
NBP1-97491F
HRP
NBP1-97491H
Janelia Fluor 525
NBP1-97491JF525
Janelia Fluor 585
NBP1-97491JF585
Janelia Fluor 635
NBP1-97491JF635
Janelia Fluor 646
NBP1-97491JF646
Janelia Fluor 669
NBP1-97491JF669
PE
NBP1-97491PE
PerCP
NBP1-97491PCP
Unconjugated
NBP1-97491-0.05mg
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Hsp47 Antibody (M16.10A1) [Janelia Fluor® 549] Summary

Immunogen
Native rat HSP47.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SERPINH1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Recognizes Sheep HSP47. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Hsp47 Antibody (M16.10A1) [Janelia Fluor® 549]

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Hsp47 Antibody
(M16.10A1) [Janelia F ...
NBP1-97491JF549
Hsp47 Antibody (M16.10A1) [Janelia Fluor® 549]
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Pm, Rb, Sh
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, KD

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Video Protocols

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Bioinformatics

Gene Symbol SERPINH1