Hsp47 Antibody (950828) [Unconjugated] Summary
Immunogen |
Chinese hamster ovary cell line CHO-derived recombinant human HSP47 Ala19-Asp412 Accession # P50454 |
Specificity |
Detects human HSP47 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with mouse HSP47 is observed.
|
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 5-25 ug/mL
- Simple Western 1 ug/mL
- Western Blot 0.1 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Hsp47 Antibody (950828) [Unconjugated]
Background
Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER),
where it is a collagen-specific molecular chaperone. In the ER, HSP47 interacts with and
stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be
associated with preterm birth and Osteogenesis Imperfecta type X. Serpin-H1 is up-regulated in several cancers
including squamous cell carcinoma, breast and prostate carcinomas. Expression in tumors drives malignant growth
and invasion by enhancing deposition of extracellular matrix proteins.
- Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
- Tasab M, et al, (2000) EMBO J. 19:22043.
- Kwon YJ, et al, (2009) Oncol Res. 18:1414.
- Zhu J, et al, (2015) Cancer Res. 75:15805.
- Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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