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Hsp47 Antibody (1D2-1A6)

Images

 
Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.
Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Immunoperoxidase of monoclonal antibody to SERPINH1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 5 ug/ml]
Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2 Analysis of SERPINH1 expression in HeLa.
Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in MCF-7.
Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in human pancreas.
Sandwich ELISA: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Detection limit for recombinant GST tagged SERPINH1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
1D2-1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Hsp47 Antibody (1D2-1A6) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SERPINH1 (AAH14623.1, 1 a.a. ~ 418 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL
Specificity
SERPINH1 - serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SERPINH1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hsp47 Antibody (1D2-1A6)

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SERPINH1
Entrez
OMIM
Uniprot