![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM423) - IHC-Prediluted [NBP2-48123] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (SPM423).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM423-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48123-img0001.jpg "Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM423) - IHC-Prediluted [NBP2-48123] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (SPM423).")
| Reactivity | Hu, Pm, Mu(-)Species Glossary |
| Applications | IHC |
| Clone | SPM423 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Activated human peripheral blood mononuclear cells |
| Localization | Cell Surface |
| Specificity | This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HLA-DRB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Hu-chromosome location: 6p21.3 Molecular weight of antigen: 28kDa (beta chain) Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HLA DRB1 Antibody (NBP2-48123)Find related products by research area.
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| Gene Symbol | HLA-DRB1 |
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