We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

Name: HLA DRB1 Antibody (SPM423) [CoraFluor&#8482; 1]
Brand: Novus Biologicals
SKU: NBP3-11552CL1
Price: 1019 USD

Question

Accepted Answer

While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Pm, Mu(-)Species Glossary
Applications	WB, Flow, ICC/IF, IHC, ICC/IF
Clone	SPM423
Clonality	Monoclonal
Host	Mouse
Conjugate	CoraFluor 1

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-11552AF350

Alexa Fluor 405 NBP3-11552AF405

Alexa Fluor 488 NBP3-11552AF488

Alexa Fluor 532 NBP3-11552AF532

Alexa Fluor 594 NBP3-11552AF594

Alexa Fluor 647 NBP3-11552AF647

Alexa Fluor 700 NBP3-11552AF700

Alexa Fluor 750 NBP3-11552AF750

Allophycocyanin NBP3-11552APC

Biotin NBP3-11552B

DyLight 350 NBP3-11552UV

DyLight 405 NBP3-11552V

DyLight 488 NBP3-11552G

DyLight 550 NBP3-11552R

DyLight 594 NBP3-11552DL594

DyLight 650 NBP3-11552C

DyLight 680 NBP3-11552FR

DyLight 755 NBP3-11552IR

FITC NBP3-11552F

HRP NBP3-11552H

Janelia Fluor 525 NBP3-11552JF525

Janelia Fluor 549 NBP3-11552JF549

Janelia Fluor 646 NBP3-11552JF646

Janelia Fluor 669 NBP3-11552JF669

PE NBP3-11552PE

PerCP NBP3-11552PCP

Unconjugated NBP2-45313-0.1mg

mFluor Violet 450 SE NBP3-11552MFV450

mFluor Violet 500 SE NBP3-11552MFV500

mFluor Violet 610 SE NBP3-11552MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP2-45313-0.1mg

IHC-Prediluted NBP2-48123

Azide and BSA Free NBP3-11552

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11552AF350
Alexa Fluor 405	NBP3-11552AF405
Alexa Fluor 488	NBP3-11552AF488
Alexa Fluor 532	NBP3-11552AF532
Alexa Fluor 594	NBP3-11552AF594
Alexa Fluor 647	NBP3-11552AF647
Alexa Fluor 700	NBP3-11552AF700
Alexa Fluor 750	NBP3-11552AF750
Allophycocyanin	NBP3-11552APC
Biotin	NBP3-11552B
DyLight 350	NBP3-11552UV
DyLight 405	NBP3-11552V
DyLight 488	NBP3-11552G
DyLight 550	NBP3-11552R
DyLight 594	NBP3-11552DL594
DyLight 650	NBP3-11552C
DyLight 680	NBP3-11552FR
DyLight 755	NBP3-11552IR
FITC	NBP3-11552F
HRP	NBP3-11552H
Janelia Fluor 525	NBP3-11552JF525
Janelia Fluor 549	NBP3-11552JF549
Janelia Fluor 646	NBP3-11552JF646
Janelia Fluor 669	NBP3-11552JF669
PE	NBP3-11552PE
PerCP	NBP3-11552PCP
Unconjugated	NBP2-45313-0.1mg
mFluor Violet 450 SE	NBP3-11552MFV450
mFluor Violet 500 SE	NBP3-11552MFV500
mFluor Violet 610 SE	NBP3-11552MFV610

HLA DRB1 Antibody (SPM423) [CoraFluor™ 1]

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Description	CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen	Activated human peripheral blood mononuclear cells
Localization	Cell Surface
Specificity	This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Isotype	IgG2b Kappa
Clonality	Monoclonal
Host	Mouse
Gene	HLA-DRB1
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunofluorescence Immunohistochemistry Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark. Do not freeze.
Buffer	PBS
Preservative	No Preservative
Purity	Protein A or G purified