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DR1 Antibody - BSA Free

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Orthogonal Strategies: Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining in human testis and liver tissues using anti-DR1 antibody. Corresponding DR1 RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human cerebral cortex, gallbladder, liver and testis using Anti-DR1 antibody NBP2-47480 (A) shows similar protein ...read more
Western Blot: DR1 Antibody [NBP2-47480] - Analysis in control (vector only transfected HEK293T lysate) and DR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DR1 Antibody [NBP2-47480] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human gallbladder.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

DR1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQEWLQMQQ
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DR1 Protein (NBP2-47480PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DR1 Antibody - BSA Free

  • Down-regulator of transcription 1
  • down-regulator of transcription 1, TBP-binding (negative cofactor 2)
  • NC2
  • NC2-BETA
  • Negative cofactor 2-beta
  • protein Dr1
  • TATA-binding protein-associated phosphoprotein

Background

DR1 encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DR1