Reactivity | Hu, Bv, FeSpecies Glossary |
Applications | Flow, ICC/IF, CyTOF-ready, ICC/IF |
Clone | 169-1B5.2 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | PE/Atto594 |
Immunogen | Human peripheral blood leukocytes (Uniprot: P01903) |
Localization | Cell surface |
Specificity | This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. |
Isotype | IgG2b Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | HLA-DRA |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. |
Storage | Store at 4C in the dark. Do not freeze. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Gene Symbol | HLA-DRA |