| Reactivity | MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse BTNL2/Butyrophilin-like 2 aa 27-452 Accession # O70355 |
| Specificity | Detects mouse BTNL2/Butyrophilin-like 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) BTN, rmBTN2, and recombinant human BTN is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | BTNL2 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Butyrophilin-like 2 (BTNL2; also BTL-II) is a predicted 60‑65 kDa member of the BTN/MOG family, Ig-superfamily of proteins. In mouse, it is expressed in intestinal villi columnar epithelial cells and colonic dendritic cells. BTNL2 apparently blocks CD4+ T cell activation. Mouse BTNL2 is a 454 amino acid (aa) type II transmembrane glycoprotein. It contains a 431 aa extracellular region (aa 24‑454) with four V-type Ig-like domains. There are three splice variants. One shows a deletion of the two C-terminal amino acids, a second shows a deletion of aa 237‑243, while a third shows a C-terminal 62 substitution for Gly453Trp454. Over aa 27‑452, mouse BTNL2 shares 88% and 64% aa identity with rat and human BTNL2, respectively.
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Secondary Antibodies |
Isotype Controls |
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