Novus Biologicals products are now on bio-techne.com

Histamine H1R Antibody

Images

 
Western Blot: Histamine H1 R Antibody [NBP1-06039] - (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NBP1-06039] - (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Histamine H1R Antibody Summary

Immunogen
Peptide with sequence QQTSVRREDKCETD corresponding to internal region according to NP_000852.1.
Epitope
QQTSVRREDKCETD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HRH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2-4ug/ml
  • Immunohistochemistry-Paraffin 2-4ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Simple Western
  • Western Blot 0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human heart lysates (calculated MW of 55.8 kDa band according to NP_000852.1).
HC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli.
Control
Histamine H1R Overexpression Lysate
Publications
Read Publications using NBP1-06039.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histamine H1R Antibody

  • H1R
  • H1-R
  • hisH1
  • Histamine H1 R
  • Histamine H1R
  • histamine receptor H1
  • HRH1
  • subclass H1

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-812
Species: Hu, Rt
Applications: Flow, ICC/IF, PEP-ELISA, WB
DY413
Species: Mu
Applications: ELISA
NLS3775
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NLS3262
Species: Bv, Eq, Gp, Ha, Hu, Pm, Mu, Pm, Rt
Applications: IHC, IHC-P, WB (-)
6507-IL/CF
Species: Hu
Applications: BA
NB600-922
Species: Ch
Applications: ELISA, IHC, IP, Single-Cell Western, WB
NBP2-16800
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
M5000
Species: Mu
Applications: ELISA
NBP1-89993
Species: Hu
Applications: IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NB100-68142
Species: Hu
Applications: Flow, ICC/IF, PEP-ELISA, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
NBP1-06039
Species: Hu
Applications: WB, Simple Western, ELISA, IHC

Publications for Histamine H1R Antibody (NBP1-06039)(2)

We have publications tested in 2 applications: Simple Western, WB.


Filter By Application
Simple Western
(1)
WB
(1)
All Applications
Filter By Species
All Species

Reviews for Histamine H1R Antibody (NBP1-06039) (0)

There are no reviews for Histamine H1R Antibody (NBP1-06039). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Histamine H1R Antibody (NBP1-06039) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Histamine H1R Products

Research Areas for Histamine H1R Antibody (NBP1-06039)

Find related products by research area.

Blogs on Histamine H1R

There are no specific blogs for Histamine H1R, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Histamine H1R Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol HRH1
Entrez
Uniprot