![Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Histamine-H2R-Antibody-Western-Blot-NB600-812-img0001.jpg "Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Rt, Mu, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QEEKPLKLQVWSGTE corresponding to C-Terminus according to NP_071640.1, NP_001124527.1. |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Predicted Species | Mouse (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | HRH2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to NP_001354640.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histamine H2R Antibody (NB600-812)Find related products by research area.
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| Gene Symbol | HRH2 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Histamine H2R Antibody [NB600-812] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Histamine-H2R-Antibody-Immunocytochemistry-Immunofluorescence-NB600-812-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histamine H2R Antibody [NB600-812] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Histamine-H2R-Antibody-Flow-Cytometry-NB600-812-img0003.jpg "Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
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