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Hemogen Antibody

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Western Blot: Hemogen Antibody [NBP1-84907] - Analysis in control (vector only transfected HEK293T lysate) and HEMGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding HEMGN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human kidney shows no positivity in cells in tubules.
Independent Antibodies: Immunohistochemistry-Paraffin: Hemogen Antibody [NBP1-84907] - Staining of human bone marrow, colon, kidney and testis using Anti-HEMGN antibody (A) NBP1-84907 shows similar protein ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Hemogen Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HEMGN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Hemogen Protein (NBP1-84907PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Hemogen Antibody

  • EDAGEDAG-1
  • Erythroid differentiation-associated gene protein
  • hemogen
  • Hemopoietic gene protein
  • NDR
  • Negative differentiation regulator protein

Background

Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol HEMGN