![Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Western-Blot-NBP2-03269-img0007.jpg "Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.")
| Reactivity | Hu, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1B3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HDAC6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 131.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
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| Publication using NBP2-03269 | Applications | Species |
|---|---|---|
| Sirchia SM, Faversani A, Rovina D et al. Epigenetic effects of chromatin remodeling agents on organotypic cultures Epigenomics 2016-03-01 [PMID: 26949823] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for HDAC6 Antibody (NBP2-03269)Find related products by research area.
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LAMP2: Protector of the lysosome LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunocytochemistry-Immunofluorescence-NBP2-03269-img0006.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human pancreas tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0005.jpg "Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human pancreas tissue using anti-HDAC6 mouse monoclonal antibody.")
![Flow Cytometry: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Flow-Cytometry-NBP2-03269-img0001.jpg "Flow Cytometry: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.")
![Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Western-Blot-NBP2-03269-img0002.jpg "Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0003.jpg "Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human liver tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0004.jpg "Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human liver tissue using anti-HDAC6 mouse monoclonal antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")