HDAC6 Antibodies
We offer HDAC6 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Negative), Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each HDAC6 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HDAC6 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat. Use the list below to choose the HDAC6 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HDAC6 polyclonal antibodies and browse our HDAC6 monoclonal antibody catalog.
Alternate Names for HDAC6 Antibodies
anti-HDAC6 antibody, anti-EC 3.5.1.98 antibody, anti-HD6FLJ16239 antibody, anti-histone deacetylase 6 antibody, anti-JM21 antibody, anti-KIAA0901 antibody
16 Results for "hdac6" in Primary Antibodies
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Western-Blot-NBP2-03269-img0007.jpg "Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunocytochemistry-Immunofluorescence-NBP2-03269-img0006.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human pancreas tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0005.jpg)
![Flow Cytometry: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Flow-Cytometry-NBP2-03269-img0001.jpg)
![Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Western-Blot-NBP2-03269-img0002.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human liver tissue using anti-HDAC6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-1B3-Immunohistochemistry-Paraffin-NBP2-03269-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78981-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Flow Cytometry: HDAC6 Antibody [NBP1-78981] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP1-78981. Staining of isolated T-cells. Image from verified customer review (Andressa Sodre, Moffitt Cancer Center).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Flow-Cytometry-NBP1-78981-img0003.jpg)
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP1-69127-img0001.jpg "Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver")
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Pineal Tissue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69127-img0004.jpg)
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Adult heart Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-NBP1-69127-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP1-69127] - Human testis tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP1-69127-img0003.jpg)
![Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP2-41286-img0004.jpg "Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP2-41286] - Human testis tissue with HDAC6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP2-41286-img0002.jpg)
![Immunohistochemistry: HDAC6 Antibody [NBP2-41286] - Human testis tissue with HDAC6 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunofluorescence-NBP2-41286-img0005.jpg)
![Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Western-Blot-NBP3-16082-img0002.jpg "Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunohistochemistry of paraffin-embedded human breast cancer using HDAC6 Rabbit mAb (NBP3-16082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Immunohistochemistry-Paraffin-NBP3-16082-img0001.jpg)
![Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC6 antibody (NBP3-16082). Western blot was performed from the immunoprecipitate using HDAC6 antibody (NBP3-16082) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Immunoprecipitation-NBP3-16082-img0003.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Immunocytochemistry-Immunofluorescence-NBP3-13824-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).")
![Flow Cytometry: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Flow-Cytometry-NBP3-13824-img0001.jpg)
![Protein Array: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC6 Antibody (PCRP-HDAC6-1A4).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Protein-Array-NBP3-13824-img0004.jpg)
![Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0004.jpg)
![Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 (lot NB100-61065-2) used for IP at 6 ug per reaction. HDAC6 was also immunoprecipitated by a previous lot of this antibody NB100-61065-1. For blotting immunoprecipitated HDAC6,NB100-61065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunoprecipitation-NB100-61065-img0009.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-82212] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82212-img0003.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-82212] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP1-82212] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP1-82212-img0011.jpg)