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GRAP2 Antibody

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse GRAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRAP2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4640-SP
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GRAP2 Antibody Summary

Immunogen
E. coli-derived recombinant human GRAP2
Met1-Arg330
Accession # O75791
Specificity
Detects endogenous human and mouse GRAP2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRAP2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRAP2 Antibody

  • Adapter protein GRID
  • GADS
  • GADSSH3-SH2-SH3 adapter Mona
  • GRAP2
  • GRB2L
  • GRB-2-like protein
  • GRB2-related adapter protein 2
  • GRB2-related adaptor protein 2
  • GRB2-related protein with insert domain
  • GRBLG
  • GRBLGGRAP-2
  • GRBX
  • Grf40 adapter protein
  • Grf40
  • grf-40
  • GRID
  • GRIDGRPL
  • Growth factor receptor-binding protein
  • growth factor receptor-bound protein 2-related adaptor protein 2
  • GRPL
  • Hematopoietic cell-associated adapter protein GrpL
  • hematopoietic cell-associated adaptor protein GRPL
  • Mona
  • P38
  • Protein GADS
  • SH3-SH2-SH3 adaptor molecule

Background

GRAP2 (GRB2 related adaptor protein 2), a divergent member of the GRB2/Sem5/Drk family, is an adaptor protein involved in leukocyte-specific protein-tyrosine kinase signaling. GRAP2 shares similar structural organization as GRB2 and GRAP with a central SH2 domain flanked by 2 SH3 domains. However, unlike GRB2 and GRAP, the SH2 domain and the C-terminal SH3 domain of GRAP2 contains a unique 120 amino acid glutamine/proline rich sequence. In T lymphocytes, GRAP2 interacts with LAT and SLP-76 to regulate NF-AT (nuclear factor of activated T cells) and form a signaling complex with HPK1 to mediate the JNK signaling pathway. GRAP2 also interacts with M-CSFR and the activated T cell co-stimulatory receptor CD28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GRAP2
Uniprot