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Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein

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SDS-Page: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405] - GPD1, 37.5 kDa (349aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
SDS-Page: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405] - GPD1, 37.5 kDa (349aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-349 of Human Glycerol 3 Phosphate Dehydrogenase

Source: E.coli

Amino Acid Sequence: MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GPD1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein

  • EC 1.1.1
  • EC 1.1.1.8
  • FLJ26652
  • glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
  • glycerol-3-phosphate dehydrogenase 1 (soluble)
  • glycerol-3-phosphate dehydrogenase, soluble
  • GPD-C
  • GPDH-C

Background

GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Recombinant GPD1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GPD1