Glutathione Reductase Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Spectrophotometer (340 nm) Assay time: 90 min |
Standard Curve Range |
6.2 - 320 U/L |
Sensitivity |
6.2 U/L |
Inter-Assay |
%CV < 2.5 |
Intra-Assay |
%CV < 2.1 |
Spike Recovery |
Average recovery rate: 100% |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
GSR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Reagent 1: Buffer Solution
- Reagent 2: Substrate
- Reagent 3: Enzyme Reagent
|
Alternate Names for Glutathione Reductase Activity Assay Kit (Colorimetric)
Background
Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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