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Glutathione Reductase Activity Assay Kit (Colorimetric)

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Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] - Standard Reference Curve
Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma, tissue and cell samples
Standard Curve Range
6.2 - 320 U/L
Sensitivity
6.2 U/L

Order Details

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Glutathione Reductase Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Spectrophotometer (340 nm)
Assay time: 90 min
Standard Curve Range
6.2 - 320 U/L
Sensitivity
6.2 U/L
Inter-Assay
%CV < 2.5
Intra-Assay
%CV < 2.1
Spike Recovery
Average recovery rate: 100%
Kit Type
Activity Assay Kit (Colorimetric)
Gene
GSR

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Reagent 1: Buffer Solution
  2. Reagent 2: Substrate
  3. Reagent 3: Enzyme Reagent

Alternate Names for Glutathione Reductase Activity Assay Kit (Colorimetric)

  • EC 1.8.1
  • EC 1.8.1.7
  • GLUR
  • Glutathione Reductase
  • glutathione reductase, mitochondrial
  • GR
  • GRase
  • GRD1
  • GSR
  • HEL-752
  • MGC78522

Background

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GSR