Human Glutathione Reductase ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human Glutathione Reductase in samples. No significant cross-reactivity or interference between Human Glutathione Reductase and analogues was observed. |
Standard Curve Range |
78.13 - 5000 pg/mL |
Sensitivity |
46.88 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
%CV < 6 |
Intra-Assay |
%CV < 6 |
Spike Recovery |
average = 97% for serum; average = 92% for EDTA plasma; average = 93% for cell culture media |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GSR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
|
Alternate Names for Human Glutathione Reductase ELISA Kit (Colorimetric)
Background
Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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