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Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

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Western Blot: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-G
Western Blot: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for ...read more
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.
Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase.
Western Blot: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Glutamine Synthetase monoclonal antibody (HepG2: human; ...read more
Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of paraffin-embedded Human breast tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of paraffin-embedded Human Ovary tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Analysis of Hela cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody ...read more
Flow Cytometry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Analysis of Jurkat cells, using anti-Glutamine Synthetase antibody, (Red), compared to a nonspecific negative control antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70834AF350
Alexa Fluor 405
NBP2-70834AF405
Alexa Fluor 488
NBP2-70834AF488
Alexa Fluor 532
NBP2-70834AF532
Alexa Fluor 594
NBP2-70834AF594
Alexa Fluor 647
NBP2-70834AF647
Alexa Fluor 700
NBP2-70834AF700
Alexa Fluor 750
NBP2-70834AF750
Allophycocyanin
NBP2-70834APC
Biotin
NBP2-70834B
CoraFluor 1
NBP2-70834CL1
DyLight 350
NBP2-70834UV
DyLight 405
NBP2-70834V
DyLight 488
NBP2-70834G
DyLight 550
NBP2-70834R
DyLight 594
NBP2-70834DL594
DyLight 650
NBP2-70834C
DyLight 680
NBP2-70834FR
DyLight 755
NBP2-70834IR
FITC
NBP2-70834F
HRP
NBP2-70834H
Janelia Fluor 525
NBP2-70834JF525
Janelia Fluor 549
NBP2-70834JF549
Janelia Fluor 646
NBP2-70834JF646
Janelia Fluor 669
NBP2-70834JF669
PE
NBP2-70834PE
PerCP
NBP2-70834PCP
Unconjugated
NBP2-02125
mFluor Violet 450 SE
NBP2-70834MFV450
mFluor Violet 500 SE
NBP2-70834MFV500
mFluor Violet 610 SE
NBP2-70834MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02125
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Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GLUL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
Theoretical MW
41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

  • cell proliferation-inducing protein 59
  • EC 4.1.1.15
  • EC 6.3.1.2
  • GLNSPIG59
  • Glutamate decarboxylase
  • glutamate-ammonia ligase (glutamine synthase)
  • glutamate-ammonia ligase
  • Glutamate--ammonia ligase
  • glutamine synthase
  • glutamine synthetase
  • GS
  • PIG43
  • proliferation-inducing protein 43

Background

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GLUL