GLUT9 Antibody Summary
Immunogen |
Synthetic peptide made to an internal portion of human GLUT9 (within residues 10-60). [Swiss-Prot# Q9NRM0] |
Localization |
Membrane (multi-pass membrane protein) |
Specificity |
This antibody is specific for the human GLUT9 protein Isoform 1 (SLC2A9-L). |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
SLC2A9 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:200 - 1:500
- Western Blot 0.5 ug/ml
|
Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
58.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control |
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Control Peptide |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
Tris-Glycine and 0.15M NaCl |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for GLUT9 Antibody
Background
GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for GLUT9 Antibody (NBP1-06565). (Showing 1 - 2 of 2 FAQ).
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I am interested in your glut-9 antibody (NBP1-06565), however I have one query I would appreciate your help with. Under specificity, it is claimed that this antibody is specific to the glut9 protein isoform A. Could you please clarify which isoform this is, as I am unfamiliar with the A nomenclature. I am aware of the isoform SLC2A9-001 (511 amino acids) and SLC2A9-002 (540 amino acids). Which isoform is GLUT9 A?
- NBP1-06565 will recognize isoform A, which is also called isoform 1. The antibody was raised against a region of the N-terminus of GLUT9 isoform A, and will not detect isoform B/2. Please see this link to uniprot.org for info http://www.uniprot.org/uniprot/Q9NRM0
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Under specificity, it is claimed that this antibody is specific to the glut9 protein isoform A. Could you please clarify which isoform this is, as I am unfamiliar with the A nomenclature. I am aware of the isoform SLC2A9-001 (511 amino acids) and SLC2A9-002 (540 amino acids). Which isoform is GLUT9 A?
- GLUT9 antibody (NBP1-06565) recognises the 511 amino acid isoform with WB bands at about 55 Kda MW position.
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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