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GLUT9 Antibody

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Western Blot: GLUT9 Antibody [NBP1-06565] - Detection of GLUT9a in human kidney membrane prep.
Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06565] - IHC-P analysis mouse kidney section with GLUT9 antibody at 1:200 dilution. This antibody gave the expected staining of SLC2A9-L isoform in the tubular ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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GLUT9 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human GLUT9 (within residues 10-60). [Swiss-Prot# Q9NRM0]
Localization
Membrane (multi-pass membrane protein)
Specificity
This antibody is specific for the human GLUT9 protein Isoform 1 (SLC2A9-L).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.5 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
58.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Placenta Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
GLUT9 Peptide (NBP1-06565PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLUT9 Antibody

  • facilitated glucose transporter member 9
  • GLUT-9
  • GLUT9Glut9
  • GLUTX
  • solute carrier family 2 (facilitated glucose transporter), member 9
  • UAQTL2
  • urate voltage-driven efflux transporter 1

Background

GLUT9 (glucose transporter 9; SLC2A9) is a multi-pass membrane transporter protein which primarily transport urate and fructose, and may also implicate in urate reabsorption by proximal tubules as well as the transportation of glucose at low rate. GLUT9 belongs to sugar transporter (TC 2.A.1.1) family and SLC2A9 gene encodes two transcripts: a long isoform (GLUT9L/SLC2A9L) and a short isoform (GLUT9S/SLC2A9S). Placenta is one of the few tissues that express both variants suggesting their role in placental hexose transport, and GLUT9 has been shown to transport both glucose and fructose with about 3-fold higher affinity for glucose. GLUT9 has emerged as a high-capacity urate transporter which influences serum uric acid levels; and GLUT9L and GLUT9S isoforms shows strong expression in the basolateral and apical membranes, respectively, of proximal renal tubular cells. GLUT9 is an important modulator responsible for the reabsorption of urate in the apical membrane of the renal proximal tubules, and loss-of-function mutations in GLUT9 gene results in functional impairment associated with renal hypouricemia type 2 (RHUC2), a disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GLUT9 Antibody (NBP1-06565). (Showing 1 - 2 of 2 FAQ).

  1. I am interested in your glut-9 antibody (NBP1-06565), however I have one query I would appreciate your help with. Under specificity, it is claimed that this antibody is specific to the glut9 protein isoform A. Could you please clarify which isoform this is, as I am unfamiliar with the A nomenclature. I am aware of the isoform SLC2A9-001 (511 amino acids) and SLC2A9-002 (540 amino acids). Which isoform is GLUT9 A?
    • NBP1-06565 will recognize isoform A, which is also called isoform 1. The antibody was raised against a region of the N-terminus of GLUT9 isoform A, and will not detect isoform B/2. Please see this link to uniprot.org for info http://www.uniprot.org/uniprot/Q9NRM0
  2. Under specificity, it is claimed that this antibody is specific to the glut9 protein isoform A. Could you please clarify which isoform this is, as I am unfamiliar with the A nomenclature. I am aware of the isoform SLC2A9-001 (511 amino acids) and SLC2A9-002 (540 amino acids). Which isoform is GLUT9 A?
    • GLUT9 antibody (NBP1-06565) recognises the 511 amino acid isoform with WB bands at about 55 Kda MW position.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC2A9