![Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0015.jpg "Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, MA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200). [UniProt P43220] |
| Localization | Cell membrane |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GLP1R |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
MiAr | Human | 12/07/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GLP-1R Antibody (NLS1205)Find related products by research area.
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![Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunocytochemistry-NLS1205-img0014.jpg "Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.")
![Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NLS1205-img0012.jpg "Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.")
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0010.jpg "Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.")
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0008.jpg "Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).")
![Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0005.jpg "Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Microarray-NLS1205-img0013.jpg "Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.")
![Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P < .05, significantly different from CSF group; #P < .05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1205_rabbit-polyclonal-glp-1r-antibody-3102024161368.jpg "Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P < .05, significantly different from CSF group; #P < .05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
