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GIPR Antibody (591853) [Unconjugated]

 (2 Publications)
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HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by ...read more
GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary ...read more
In a functional flow cytometry test, biotinylatedrecombinant Human GIP (Catalog # 2257,25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line).Binding is completely blocked (orange histogram) by 2.5 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
591853
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8210U-100UG
Alexa Fluor 405
FAB8210V-100UG
Alexa Fluor 488
FAB8210G-100UG
Alexa Fluor 532
FAB8210X
Alexa Fluor 594
FAB8210T-100UG
Alexa Fluor 647
FAB8210R-100
Alexa Fluor 700
FAB8210N-100UG
Alexa Fluor 750
FAB8210S-100UG
Allophycocyanin
FAB8210A
Allophycocyanin/Cy7
FAB8210APCCY7
Biotin
FAB8210B
DyLight 350
FAB8210D
DyLight 405
FAB8210E
DyLight 488
FAB8210K
DyLight 550
FAB8210L
DyLight 594
FAB8210M
DyLight 650
FAB8210W
DyLight 680
FAB8210Y
DyLight 755
FAB8210Z
FITC
FAB8210F
HRP
FAB8210H
Janelia Fluor 549
FAB8210I
Janelia Fluor 646
FAB8210J
PE/Atto594
FAB8210PEATT594
PE/Cy5.5
FAB8210PECY55
PE/Cy7
FAB8210PECY7
PerCP
FAB8210C
Phycoerythrin
FAB8210P-100
mFluor Violet 450 SE
FAB8210MFV450
mFluor Violet 500 SE
FAB8210MFV500
mFluor Violet 610 SE
FAB8210MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8210-SP
Carrier Free
FAB8210P-100
Carrier Free
FAB8210R-100
Carrier Free
FAB8210V-100UG
Carrier Free
FAB8210U-100UG
Carrier Free
FAB8210T-100UG
Carrier Free
FAB8210S-100UG
Carrier Free
FAB8210N-100UG
Carrier Free
FAB8210G-100UG
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GIPR Antibody (591853) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human GIPR.
Met1-Cys466
Accession # P48546
Specificity
Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GIPR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction 2.5 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB8210 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GIPR Antibody (591853) [Unconjugated]

  • gastric inhibitory polypeptide receptor
  • GIPR
  • GIP-R
  • Glucose-dependent insulinotropic polypeptide receptor
  • MGC126722
  • PGQTL2

Background

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GIPR Antibody (MAB8210)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Bioinformatics

Gene Symbol GIPR
Uniprot